Ryann Joseph

Education

Harvard University

Ph.D. in Bioengineering in the School of Engineering and Applied Science.

University of Pennsylvania

BSE in Bioengineering in the School of Engineering and Applied Science.
Minors in mathematics and chemistry.

The Ohio State University

Enriched high school education by completing coursework in calculus, chemistry, and Spanish.

Olentangy Liberty High School

Graduated valedictorian ranked 1/509 with weighted GPA 4.611/4.000.
Relevant activities include marching band (drum major), cheerleading, lacrosse (captain), Science Olympiad.

Research Experience

Mooney Lab: Laboratory for Cell and Tissue Engineering

PhD student in the Mooney lab with projects relating to drug delivery, cancer immunotherapy, and nanomaterials.

Mitchell Lab: Overcoming Biological Barriers to Drug Delivery

Research Assistant in the Mitchell Lab, collaborating with graduate bioengineers to maximize drug delivery intracellularly via LNPs.
Pioneered projects for Pfizer for solid tumor delivery and for the Cystic Fibrosis Foundation for novel therapeutic gene editing.

Publications

1. Elucidating lipid nanoparticle properties and structure through biophysical analyses
   Marshall S. Padilla, Sarah J. Shepherd, Andrew R. Hanna, Martin Kurnik, Xujun Zhang, Michelle Chen, James Byrnes, Ryann A. Joseph, Hannah M. Yamagata, Adele S. Ricciardi, Kaitlin Mrksich, David Issadore, Kushol Gupta & Michael J. Mitchell
   Nature Biotechnology 2025
   10.1038/s41587-025-02855-x
2. Cas9 protein outperforms mRNA in lipid nanoparticle-mediated CFTR repair
   Ryann A. Joseph, Rebecca M. Haley, Marshall S. Padilla, Adele S. Ricciardi, Hannah M. Yamagata, Michael J. Mitchell
   Nano Letters 2025
   10.1021/acs.nanolett.5c03548
3. Ionizable lipid nanoparticles with functionalized PEG-lipids increase retention in the tumor microenvironment
   Matthew Jester, Rebecca Haley, Margaret Billingsley, Christian Figueroa-Espada, Ryann Joseph, Xuexiang Han, Michael Mitchell
   Molecular Therapy-Methods & Clinical Development 2025 10.1016/j.omtm.2025.101457
4. Lipid Nanoparticles for In Vivo Lung Delivery of CRISPR-Cas9 Ribonucleoproteins Allow Gene Editing of Clinical Targets
   Rebecca M Haley, Marshall S Padilla, Rakan El-Mayta, Ryann A Joseph, Jesse A Weber, Christian G Figueroa-Espada, Alvin J Mukalel, Adele S Ricciardi, Rohan Palanki, Hannah C Geisler, Matthew T Jester, Beverly L Davidson, Michael J Mitchell
   ACS Nano 2025 10.1021/acsnano.4c16617
5. Placenta-tropic VEGF mRNA lipid nanoparticles ameliorate murine pre-eclampsia
   Kelsey L. Swingle, Alex G. Hamilton, Hannah C. Safford, Hannah C. Geisler, Ajay S. Thatte, Rohan Palanki, Amanda M. Murray, Emily L. Han, Alvin J. Mukalel, Xuexiang Han, Ryann A. Joseph, Aditi A. Ghalsasi, Mohamad-Gabriel Alameh, Drew Weissman, Michael J. Mitchell
   Nature 2024 10.1038/s41586-024-08291-2
6. Lipid nanoparticle-mediated RNA delivery for immune cell modulation
   Emily H. Kim, Sridatta V. Teerdhala, Marshall S. Padilla, Ryann A. Joseph, Jacqueline J. Li, Rebecca M. Haley, Michael J. Mitchell
   European Journal of Immunology 2024 10.1002/eji.202451008
7. High-Throughput In Vivo Screening Identifies Differential Influences on mRNA Lipid Nanoparticle Immune Cell Delivery by Administration Route
   Alex G. Hamilton, Kelsey L. Swingle, Ajay S. Thatte, Alvin J. Mukalel, Hannah C. Safford, Margaret M. Billingsley, Rakan D. El-Mayta, Xuexiang Han, Benjamin E. Nachod, Ryann A. Joseph, Ann E. Metzloff, Michael J. Mitchell
   ACS Nano 2024 10.1021/acsnano.4c01171
8. Influence of ionizable lipid tail length on lipid nanoparticle delivery of mRNA of varying length
   Kaitlin Mrksich, Marshall S. Padilla, Ryann A. Joseph, Emily L. Han, Dongyoon Kim, Rohan Palanki, Junchao Xu, Michael J. Mitchell
   Journal of Biomedical Materials Research 2024 10.1002/jbm.a.37705
9. Ionizable lipid nanoparticles with integrated immune checkpoint inhibition for mRNA CAR T cell engineering
   Alex G. Hamilton, Kelsey L. Swingle, Ryann A. Joseph, David Mai, Ningqiang Gong, Margaret M. Billingsley, Mohamad-Gabriel Alameh, Drew Weissman, Neil C. Sheppard, Carl H. June, Michael J. Mitchell
   Advanced Healthcare Materials 2023 10.1002/adhm.202301515
10. Ionizable lipid nanoparticles for in vivo mRNA delivery to the placenta during pregnancy
    Kelsey L. Swingle, Hannah C. Safford, Hannah C. Geisler, Alex G. Hamilton, Ajay S. Thatte, Margaret M. Billingsley, Ryann A. Joseph, Kaitlin Mrksich, Marshall S. Padilla, Aditi A. Ghalsasi, Mohamad-Gabriel Alameh, Drew Weissman, Michael J. Mitchell
    Journal of the American Chemical Society 2023 145 (8), 4691-4706

Presentations

1. Comparing lipid nanoparticle mediated delivery of Cas9 mRNA and protein for cystic fibrosis CRISPR gene editing
   Ryann A. Joseph, Rebecca M. Haley, Adele S. Riccardi, Marshall S. Padilla, Michael J. Mitchell, Oral presentation
   Biomedical Engineering Society Annual Meeting, Baltimore, MD (2024)
2. Comparing lipid nanoparticle mediated delivery of Cas9 mRNA and protein for cystic fibrosis CRISPR gene editing
   Ryann A. Joseph, Rebecca M. Haley, Adele S. Riccardi, Marshall S. Padilla, Michael J. Mitchell, Poster presentation
   Center for Undergraduate Research and Fellowships Fall Research Expo, University of Pennsylvania (2024)
3. Comparing lipid nanoparticle mediated delivery of Cas9 mRNA and protein for cystic fibrosis CRISPR gene editing
   Ryann A. Joseph, Rebecca M. Haley, Adele S. Riccardi, Marshall S. Padilla, Michael J. Mitchell, Poster presentation
   Undergraduate Summer Research Expo, University of Pennsylvania (2024)
4. Synthesis of Barcoded mRNA for High-Throughput Nucleic Acid Delivery Screening
   Ryann A. Joseph, Alex G. Hamilton, Michael J. Mitchell, Poster presentation
   Center for Undergraduate Research and Fellowships Fall Research Expo, University of Pennsylvania (2022)

Work Experience

Bioengineering Laboratory Teaching Assistant

Advise and educate second year bioengineering students in introductory medical device development  (BE 2700).

Mathematics Teaching Assistant

Lead instruction and assist in the education of students studying linear algebra and ordinary differential equations (MATH 2400).

Team Manager

Managed the functions of a food service establishment while assisting customers.

Instructor

Facilitated the mathematics education of students from ages five to eighteen.

Leadership and Activities

Awards

SEAS Senior Design Judge’s Choice Award (2025)^{read more!}

Rose Undergraduate Reseach Award (2025)^{read more!}

Penn Engineering Exceptional Service Award (2025)^{read more!}

Mathematics Departmental Good Teaching Award (Fall 2024)

Mathematics Departmental Good Teaching Award (Spring 2024)

Advancing Women in Engineering Undergraduate Summer Research Award (2024)

Jumpstart for Juniors Award (2024)

Penn Undergraduate Research and Mentorship (PURM) Fellow (2022)

High School Valedictorian (2021)

Ohio Seal of Biliteracy (2021)

Skills

Leadership – high level of experience with leading peers in marching band, student government, retail employment, and more.

Customer Service – comprehensive experience with management of a retail business and its patrons.

Computer – Python (proficient), Java (introductory), LaTeX (proficient), Microsoft (Word, Excel) (proficient), Google (Sheets, Docs) (proficient).

Laboratory – General lab and molecular biology skills (cell culture, gel electrophoresis, PCR, IVT, dissection, CRISPR-Cas9, flow cytometry)